Primary Structure of Proteins

Protein Structure

Recall that the shape of a protein is critical to its function. For example, an enzyme can bind to a specific substrate at a site we refer to as the active site. If local changes or changes in overall protein structure alter this active site, the enzyme may be unable to bind to the substrate.

In order to understand how the protein gets its final shape or conformation, we need to understand the four levels of protein structure. Generally, these levels are primary, secondary, tertiary, and quaternary.

Primary Structure

The unique sequence of amino acids in a polypeptide chain is its primary structure. For example, the pancreatic hormone insulin has two polypeptide chains, A and B. These chains are linked together by disulfide bonds. The N terminal amino acid of the A chain is glycine. On the other hand, the C terminal amino acid is asparagine (see image below). The sequences of amino acids in the A and B chains are unique to insulin.

insulin-protein

Bovine serum insulin is a protein hormone made of two peptide chains. The chains are A (21 amino acids long) and B (30 amino acids long). In each chain, primary structure is indicated by three-letter abbreviations. Basically, the abbreviations represent the names of the amino acids in the order they are present. The amino acid cysteine (cys) has a sulfhydryl (SH) group as a side chain. Two sulfhydryl groups can react in the presence of oxygen to form a disulfide (S-S) bond. Two disulfide bonds connect the A and B chains together, and a third helps the A chain fold into the correct shape. Note that all disulfide bonds are the same length, but are drawn different sizes for clarity. Image Attribution: OpenStax Biology

The gene encoding every protein ultimately determines the unique sequence for the protein. A change in nucleotide sequence of the gene’s coding region may lead to a different amino acid being added to the growing polypeptide chain. This causes a change in protein structure and function.

Hemoglobin molecule and sickle cell anemia

In sickle cell anemia, the hemoglobin β chain (a small portion of which you can see in the image below) has a single amino acid substitution. This causes a change in protein structure and function. Specifically, valine substitutes the amino acid glutamic acid in the β chain.

hemoglobin-beta-chain

The beta chain of hemoglobin is 147 residues in length, yet a single amino acid substitution leads to sickle cell anemia. In normal hemoglobin, the amino acid at position seven is glutamate. In sickle cell hemoglobin, this glutamate is replaced by a valine. Image Attribution: OpenStax Biology

What is most remarkable to consider is that a hemoglobin molecule is made up of two alpha chains and two beta chains that each consist of about 150 amino acids. The molecule, therefore, has about 600 amino acids. The structural difference between a normal hemoglobin molecule and a sickle cell molecule—which dramatically decreases life expectancy—is a single amino acid of the 600. What is even more remarkable is that three nucleotides encode each of those 600 amino acids. But a single base change (point mutation), 1 in 1800 bases causes the mutation.

normal-cells-vs-sickle-cells

In this blood smear, visualized at 535x magnification using bright field microscopy, sickle cells are crescent shaped (somewhat semicircular). Normal cells on the other hand, are disc-shaped. Image Attribution: Modification of work by Ed Uthman; scale-bar data from Matt Russell

Because of this change of one amino acid in the chain, hemoglobin molecules form long fibers that distort the biconcave, or disc-shaped, red blood cells and assume a crescent or “sickle” shape, which clogs arteries (see image above). This can lead to myriad serious health problems such as breathlessness, dizziness, headaches, and abdominal pain for those affected by this disease.

[Attributions and Licenses]


This is a lesson from the tutorial, Biological Macromolecules and you are encouraged to log in or register, so that you can track your progress.

Log In

Share Thoughts