# Medicine: The Buffer System in Blood

## Medicine: The Buffer System in Blood

The normal pH of human blood is about 7.4. The carbonate buffer system in the blood uses the following equilibrium reaction:

$${\text{CO}}_{2}\left(g\right)+2{\text{H}}_{2}\text{O}\left(l\right)\phantom{\rule{0.2em}{0ex}}⇌\phantom{\rule{0.2em}{0ex}}{\text{H}}_{2}{\text{CO}}_{3}\left(aq\right)\phantom{\rule{0.2em}{0ex}}⇌\phantom{\rule{0.2em}{0ex}}{\text{HCO}}_{3}{}^{\text{−}}\left(aq\right)+{\text{H}}_{3}{\text{O}}^{\text{+}}\left(aq\right)$$

The concentration of carbonic acid, H2CO3 is approximately 0.0012 M, and the concentration of the hydrogen carbonate ion, $${\text{HCO}}_{3}{}^{\text{−}},$$ is around 0.024 M. Using the Henderson-Hasselbalch equation and the pKa of carbonic acid at body temperature, we can calculate the pH of blood:

$$\text{pH}=\text{p}{K}_{\text{a}}+\text{log}\phantom{\rule{0.4em}{0ex}}\cfrac{\left[\text{base}\right]}{\left[\text{acid}\right]}\phantom{\rule{0.2em}{0ex}}=6.4+\text{log}\phantom{\rule{0.4em}{0ex}}\cfrac{0.024}{0.0012}\phantom{\rule{0.2em}{0ex}}=7.7$$

The fact that the H2CO3 concentration is significantly lower than that of the $${\text{HCO}}_{3}{}^{\text{−}}$$ ion may seem unusual, but this imbalance is due to the fact that most of the by-products of our metabolism that enter our bloodstream are acidic. Therefore, there must be a larger proportion of base than acid, so that the capacity of the buffer will not be exceeded.

Lactic acid is produced in our muscles when we exercise. As the lactic acid enters the bloodstream, it is neutralized by the $${\text{HCO}}_{3}{}^{\text{−}}$$ ion, producing H2CO3. An enzyme then accelerates the breakdown of the excess carbonic acid to carbon dioxide and water, which can be eliminated by breathing. In fact, in addition to the regulating effects of the carbonate buffering system on the pH of blood, the body uses breathing to regulate blood pH. If the pH of the blood decreases too far, an increase in breathing removes CO2 from the blood through the lungs driving the equilibrium reaction such that [H3O+] is lowered. If the blood is too alkaline, a lower breath rate increases CO2 concentration in the blood, driving the equilibrium reaction the other way, increasing [H+] and restoring an appropriate pH.

### Optional Video:

Watch this video to view information on the buffer system encountered in natural waters.